NADome and Associated Nucleotide Metabolism
Select a compound below to view its biological context, or open the pathway graphic to explore salvage routes and the two nicotinamide redox pairs.
NAD Biosynthesis and Redox Pathway
Solid arrows represent biochemical routes or interconversions; they do not imply measurement of every substrate or intermediate. Selected metabolites are highlighted in gold.
Nicotinamide salvage and NAD biosynthesis
Distinct entry routes converge on the NAD pool.
NAMPT: NAM → NMN; NMNAT: NMN + ATP → NAD + PPi
NR kinase phosphorylates NR to NMN.
NAD synthetase amidates NAAD to NAD.
Distinct NAD and NADP redox pools
The NADP branch originates from NAD⁺ phosphorylation, not NADH.
NAD⁺/NADH redox couple
NADP⁺/NADPH redox couple
ATP as a biosynthetic substrate
ATP is measured as a nucleotide, not an NAD-family cofactor.
The graphic is intentionally simplified. ATP and unmeasured substrates participate in multiple reactions, and NAD levels or ratios alone do not establish metabolic flux.
Interpret NAD/NADH and NADP/NADPH concentrations with attention to sampling, extraction, oxidation state stability, and sample matrix. Compounds are assay targets; measured abundance does not establish pathway flux.