FAMEs assay analytes and pathway relationships
Select any of the 36 measured FAME species to view its fatty-acid identity and biological context. Open the pathway graphic to explore family and conversion relationships.
Measurement scopeThe assay measures total fatty acids, including free fatty acids (FFAs) and esterified fatty acids in glycerolipids (mono-, di-, and triglycerides) and glycerophospholipids (e.g., phosphatidylcholines and phosphatidylethanolamines).
FAMEs fatty-acid families, elongation, and desaturation
Gold nodes identify all 36 measured methyl esters. Solid arrows show representative reactions between the underlying fatty-acyl species; dashed arrows indicate multi-step relationships. Separate cis/trans isomers and odd-chain species are not assumed to interconvert.
Representative conversion Multi-step or indirect relationship
The network is a simplified guide to the metabolism of the underlying fatty acids, not the derivatized methyl esters. Isomeric forms are analytically distinct. Some branch relationships require unmeasured intermediates, and the map does not establish metabolic flux.
Selected fatty acid
Biochemical contextRelationship to the measured panelExplore in PubChem ↗
All reported species reflect the FAME-based total-fatty-acid workflow. This assay does not separately quantify fatty-acid pools by lipid class or esterification state.
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